Anti-Staphylococcus aureus activity of Lactobacillus rhamnosus and Lactobacillus acidophilus on clinical isolates- an in vitro study
Year: 2024
Type: article
Abstract: Background: This study evaluated the in vitro antimicrobial and antagonistic activity of both neat and pH-adjusted whole-cell suspensions of L. rhamnosus and L. acidophilus towards S. aureus , as an alternative to control the spread of MRSA, which is endemic in India. Materials and Methods: The study was conducted at School of Medical Education, Kerala, India, between October 2022 and May 2023. Anti- Staphylococcus aureus activity of Lactobacillus rhamnosus MTCC 1408 and Lactobacillus acidophilus MTCC 10307 grown on MRS agar, was evaluated on a total of 76 clinical isolates of S. aureus from Kerala, India, by the Agar overlay method. The study involved a comparison of probiotic activities by both neat and pH-adjusted whole-cell suspensions of L. rhamnosus and L. acidophilus . Results: The study results indicate that the mean value of inhibition zones produced by L. rhamnosus was greater than that of L. acidophilus , and statistically significant data was obtained in which the pH-adjusted whole cell suspensions of L. rhamnosus exhibited larger inhibition zones than that of neat suspensions of the same. Also, the mean value of activity by pH-adjusted L. acidophilus suspension was slightly greater than its neat suspension. Conclusions: L. rhamnosus and L. acidophilus were found to possess in vitro anti-Staphylococcus aureus activity. The antimicrobial and antagonistic activity of pH-adjusted live suspensions suggests the possible use of L. acidophilus and L. rhamnosus as probiotic treatment options in sites other than the gastrointestinal tract, supporting their use as local administration and topical applications, in particular for the treatment and decolonization of S. aureus .
Source: The Journal of Medical Research
Authors: Raj Ananthu, K S Harish Kumar, Anil Anju, M M Azhar, Kuriakose Nithyamol
Institution: Centre for Professional and Advanced Studies
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4395092447