Year: 2024
Type: data-paper
Abstract: The genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes), whose members can encode useful complex traits such as solvent production, gas-fermentation, and lignocellulose breakdown. We describe 270 genome sequences of solventogenic clostridia from a comprehensive industrial strain collection assembled by Professor David Jones that includes 194 C. beijerinckii, 57 C. saccharobutylicum, 4 C. saccharoperbutylacetonicum, 5 C. butyricum, 7 C. acetobutylicum, and 3 C. tetanomorphum genomes. We report methods, analyses and characterization for phylogeny, key attributes, core biosynthetic genes, secondary metabolites, plasmids, prophage/CRISPR diversity, cellulosomes and quorum sensing for the 6 species. The expanded genomic data described here will facilitate engineering of solvent-producing clostridia as well as non-model microorganisms with innately desirable traits. Sequences could be applied in conventional platform biocatalysts such as yeast or Escherichia coli for enhanced chemical production. Recently, gene sequences from this collection were used to engineer Clostridium autoethanogenum, a gas-fermenting autotrophic acetogen, for continuous acetone or isopropanol production, as well as butanol, butanoic acid, hexanol and hexanoic acid production.
Source: Scientific Data
Authors: Rasmus O. Jensen, Frederik Schulz, Simon Roux, Dawn M. Klingeman, Wayne P. Mitchell, Daniel Udwary, Sarah Moraïs, Vinicio Reynoso, James Winkler, Shilpa Nagaraju, Sashini De Tissera, Nicole Shapiro, Natalia Ivanova, T. B. K. Reddy, Itzhak Mizrahi, Sagar M. Utturkar, Edward A. Bayer, Tanja Woyke, Nigel J. Mouncey, Michael C. Jewett, Séan D. Simpson, Michael Köpke, David T. Jones, Steven D. Brown
Institutions: Lawrence Berkeley National Laboratory, Joint Genome Institute, Oak Ridge National Laboratory, Ben-Gurion University of the Negev, Purdue University West Lafayette, Purdue University Institute for Cancer Research, Weizmann Institute of Science, University of California, Merced, Stanford University, University of Otago
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,290
Awards: G1026679911, G1122898986, G121915436, G1286236842, G1337544421, G1645119126, G166562317, G203625172, G2135731044, G223002938, G2293800358, G2710243964, G2988778103, G3083819904, G3121793570, G313676650, ESTABLISHING A CLOSTRIDIA FOUNDRY FOR BIOSYSTEMS DESIGN BY INTEGRATING COMPUTATIONAL MODELING, SYSTEMS-LEVEL ANALYSES, AND CELL-FREE ENGINEERING TECHNOLOGIES, G419881185, G42916606, G4423657506, G4501827968, G450780475, G4661815001, ESTABLISHING A CLOSTRIDIA FOUNDRY FOR BIOSYSTEMS DESIGN BY INTEGRATING COMPUTATIONAL MODELING, SYSTEMS-LEVEL ANALYSES, AND CELL-FREE ENGINEERING TECHNOLOGIES, G498139845, G5286455319, G5726405315, G6519249983, G660579501, G675320460, G7205239661, G7787287669, G8269158468, G834277795, DEVELOPMENT OF A SUSTAINABLE GREEN CHEMISTRY PLATFORM FOR, G8906985441, G8943143067, G969889393
OpenAlex ID: W4396553823