γδ T cell profiling in a cohort of preterm infants reveals elevated frequencies of CD83 + γδ T cells in sepsis
Year: 2024
Type: article
Study design: Observational Study
Abstract: Preterm infants are at high risk of developing neonatal sepsis. γδ T cells are thought to be an important set of effector cells in neonates. Here, γδ T cells were investigated in a longitudinal cohort of preterm neonates using next-generation sequencing, flow cytometry, and functional assays. During the first year of life, the Vγ9Vδ2 T cell subset showed dynamic phenotypic changes and elevated levels of fetal-derived Vγ9Vδ2 T cells were evident in infants with sepsis. Single-cell transcriptomics identified HLA-DRhiCD83+ γδ T cells in neonatal sepsis, which expressed genes related to antigen presentation. In vitro assays showed that CD83 was expressed on activated Vγ9Vδ2 T cells in preterm and term neonates, but not in adults. In contrast, activation of adult Vγ9Vδ2 T cells enhanced CD86 expression, which was presumably the key receptor to induce CD4 T cell proliferation. Together, we provide a map of the maturation of γδ T cells after preterm birth and highlight their phenotypic diversity in infections.
DOI: 10.1084/jem.20231987
Authors: Ximena León-Lara, Alina S. Fichtner, Maike Willers, Tao Yang, Katharina Schaper, Lennart Riemann, Jennifer Schöning, Anna Harms, Vicente Almeida, Anja Schimrock, Anika Janssen, Laura Ospina-Quintero, Constantin von Kaisenberg, Reinhold Förster, Matthias Eberl, Manuela F. Richter, Sabine Pirr, Dorothee Viemann, Sarina Ravens
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1284162446, Innate pathogen sensing by local unconventional T cells during microbial infections, G2157178526, G2226533489, PROSPER - Prävention von Sepsis in gefährdeten Neugeborenen durch personalisierte Nahrungssupplementierung von S100A8/A9- Untersuchung der Effektivität und Unbedenklichkeit von S100A8/A9 im Sepsis-Mausmodell und Identifizierung Sepsis-protektiver Komponenten in Bioproben etablierter Frühgeborenen-Kohorten, G3912871286, SFB 1583: Entscheidungsprozesse bei Infektionskrankheiten (DECIDE), EXC 2155: RESIST Resolving Infection Susceptibility, G4613543483, TRR 359: Perinatale Entwicklung der ImmunzellTopologie (PILOT), G5186833673, PROSPER -Prävention von Sepsis in gefährdeten Neugeborenen durch personalisierte Nahrungssupplementierung von S100A8/A9-Untersuchung und Bestimmung Sepsis-protektiver Komponenten in Bioproben etablierter Frühgeborenen-Kohorten, G6357718487, G644306628, G6775381568
OpenAlex ID: W4396977071