Year: 2024
Type: conference-paper
Abstract: Dirofilariasis is a relatively new medical problem directly related to changes in natural, climatic, and social conditions. Despite significant improvements and quality of deworming measures for dogs, the most epidemiologically significant source of Dirofilaria infection, there has been an increase in recent decades in the incidence of these nematodes in definitive hosts in Europe, both in highly endemic areas where their constant circulation is noted historically and Dirofilaria expansion into new areas. For 2023, venous blood was collected from 233 dogs from animal shelters in Rostov-on-Don and the Rostov Region. The results of the studies showed that Dirofilaria larvae were found in 53 out of 233 blood samples of the dogs by larvoscopy with an invasion intensity of one to one hundred larvae in 0.2 mL of blood, and the prevalence of 22.75%. Dirofilaria DNA was detected in 60 samples by PCR. The prevalence in the dogs was 25.75% of which Dirofilaria repens was 21.67%, D. immitis was 41.67%, and mixed invasion was 36.67%. An analysis of the results in the confusion matrix showed that the PCR sensitivity was 90.57%, and the microscopy sensitivity was 81.36%. The specificity of the first method was 93.33%, and the second method, 97.13%. The test system we are developing allows us to determine the presence of two Dirofilaria species in one study and can be implemented in research laboratories’ work.
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4397013442