Work

Replication Data for "Using TrackMate to Analyze Drosophila Larval and Adult Locomotion"

Year: 2021

Type: dataset

Abstract: Drosophila adult and larvae exhibit sophisticated behaviors that are widely used in development, synaptic transmission, sensory physiology, and learning and memory research. Many of these behaviors depend on locomotion, the ability of an animal to move. However, the statistical analysis of locomotion is not trivial. Here we use an open-source Fiji plugin TrackMate to track the locomotion of Drosophila adults and larvae. We build optimal experimental setups to rapidly process recordings by Fiji and analyze by TrackMate. We also provide tips for analyzing non-optimal recordings. TrackMate extracts the X and Y positions of an animal on each frame of an image sequence or a video. This information allows for generating moving trajectories, calculating moving distances, and determining preference indices in two-choice assays. Notably, this free-cost analysis method does not require programming skills.

Source: Harvard Dataverse

DOI: 10.7910/dvn/snbqc2

Authors: A. Omelchenko, Alisa, Ainul Huda, N. Castaneda, Allison, J. Vaden, Thomas, Ni, Lina

Institution: Virginia Tech

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4398801136

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