Work

Biological Control of Xylotrechus Rusticus (coleoptera: Cerambycidae) by Releasing of Dastarcus Helophoroides (coleoptera: Bothrideridae) and Its Expression of Cytochrome P450 Genes

Year: 2024

Type: article

Abstract: Xylotrechus rusticus is one of the major forest trunk borers in China, causing a significant threat to afforestation and forestry engineering construction.Controlling X. rusticus is challenging because of its hidden life habit.The use of natural enemies against X. rusticus is an important strategy for controlling Cerambycidae pests.This is the first study to investigate the effectiveness of Dastarcus helophoroides as a biological control against X. rusticus under indoor and outdoor environments.The expression patterns of CYP6BK3 and CYP6BQ13 genes in various tissues and at different development stages were analyzed to clarify their role in the development of D. helophoroides.The results showed that the parasitism rate of D. helophoroides was the higher when their eggs were released on the larvae of X. rusticus L. indoors.Furthermore, the parasitism rate of D. helophoroides was the highest (86.67%) when their eggs were released on the larvae of X. rusticus in the ratio of 1:100 (host: D. helophoroides) under artificial wood segment simulation.The average parasitism rates of D. helophoroides eggs and adults on the exposed wood segment of the Cerambycidae were 81.83% and 83.29%, respectively.Indoor release of Scleroderma guani increased their parasitic rate on the aged larvae and pupae of X. rusticus.qPCR analysis of CYP450 gene expression showed that CYP6BK3 and CYP6BQ13 genes were expressed at all development stages of D. helophoroides; however, their expression levels were higher in adults than in the larvae.Notably, CYP6BK3 expression was the highest in the adipose tissue, whereas CYP6BQ13 was the highest in the hindgut tissue.

Source: Applied Ecology and Environmental Research

DOI: 10.15666/aeer/2203_23772388

Author: J.N. DING

Institution: Harbin University

Language: English

Topic: Forest Insect Ecology and Management

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

SDGs: Life on land, Zero hunger

Open Access status: diamond

Funder: Natural Science Foundation of Heilongjiang Province