Genome-scale CRISPR-Cas9 screen identifies host factors as potential therapeutic targets for SARS-CoV-2 infection
Year: 2024
Type: article
Abstract: Although many host factors important for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been reported, the mechanisms by which the virus interacts with host cells remain elusive. Here, we identified tripartite motif containing (TRIM) 28, TRIM33, euchromatic histone lysine methyltransferase (EHMT) 1, and EHMT2 as proviral factors involved in SARS-CoV-2 infection by CRISPR-Cas9 screening. Our result suggested that TRIM28 may play a role in viral particle formation and that TRIM33, EHMT1, and EHMT2 may be involved in viral transcription and replication. UNC0642, a compound that specifically inhibits the methyltransferase activity of EHMT1/2, strikingly suppressed SARS-CoV-2 growth in cultured cells and reduced disease severity in a hamster infection model. This study suggests that EHMT1/2 may be a therapeutic target for SARS-CoV-2 infection.
Source: iScience
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,150
Funders: Japan Agency for Medical Research and Development, Ministry of Education, Culture, Sports, Science and Technology, Japan Society for the Promotion of Science, Japan Science and Technology Agency, RIKEN, Core Research for Evolutional Science and Technology, Program for Creating STart-ups from Advanced Research and Technology
Awards: Development of a self-editing RNA viral vector to accelerate gene therapy for neurological diseases, G1665215348, Risk assessment and pathogenicity of highly pathogenic emerging bornavirus, G2262840760, Exploring epigenomic perturbation by an episomal RNA virus vector, G3082712080, ウイルス由来エピソーマルRNAを用いた革新的幹細胞遺伝子治療ベクターの創出, G4635277790, G5672461341, ウイルス間の相互作用による感染維持メカニズム, オルガノイドを用いた呼吸器チップの開発とウイルス病態解析への応用, G8563024251
OpenAlex ID: W4400433487