Work

Genotypic and allelic variability of the calpain CAPN1-316 gene in the Tropical Milking criollo breed and indication of its meat tenderness

Year: 2024

Type: article

Abstract: Background: Genes and their variants related to milk and beef production in cattle can be identified through molecular markers. In particular, the Calpain gene CAPN1-316 has been linked to meat tenderness. The males of the Tropical Milking Criollo cattle not assigned as breeding animals are destined for commercial beef production. Objective: To estimate the genotypic and allelic frequencies in the calpain gene CAPN1-316 and its relation to meat tenderness in the Tropical Milking criollo breed. Methods: Molecular marker microsatellites of single sequence repeats (SSR) from DNA were extracted from blood samples of 423 purebred animals from three farms to identify the variants of the CAPN1-316 gene using the polymerase chain reaction (PCR). Genotypic and Allelic frequencies within populations, genetic distance, heterozygosity, and Xi squared (X2) test were performed using Pop Gene V.4. Results: The genotypic frequencies of the whole population were CC 0.31, GG 0.18, and CG 0.51. The allelic frequencies of variant C per farm were 0.62, 0.60, and 0.52. The genotypic and allelic frequencies of the CAPN1-316 polymorphisms were in Hardy-Weinberg equilibrium (X2, p>0.05). The CC genotype showed indication of greater tenderness. Conclusion: In the Tropical Milking criollo cattle, the C variant of the CAPN1-316, favorable to meat tenderness, had a bigger frequency than the G variant.

Source: Revista Colombiana de Ciencias Pecuarias

DOI: 10.17533/udea.rccp.v38n1a1