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Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing

Year: 2024
Type: article
Abstract: Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70-90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the LDL receptor located on the producer cell membrane, allowing lentiviral vector transduction. Avoiding retro-transduction in LVV manufacturing is crucial to improve net production and, therefore, the efficiency of the production process. Here, we describe a method for quantifying the transduction of producer cells and three different strategies that, focused on the interaction between VSV-G and the LDLR, aim to reduce retro-transduction.
Source: Viruses
DOI: 10.3390/v16081216
Authors: Soledad Banos-Mateos, Carlos Lopez-Robles, María Eugenia Yubero, Aroa Jurado, Ane Arbelaiz-Sarasola, Andrés Lamsfus-Calle, Ane Arrasate, Carmen Albo, Juan Carlos Ramírez, Marie J. Fertin
Language: English
Subfield: Genetics
Open Access status: gold
Estimated APC paid: $2,955
Awards: G2144323918, G273656033, G3991522474, G5324474795, G5999808631, G7002702108, G7868881876, G875069496, G8933968214
OpenAlex ID: W4401068554
FWCI: 2.012
Cites: 42
Cited by: 10
Related to: 10