Mitochondrial-derived compartments are multilamellar domains that encase membrane cargo and cytosol
Year: 2024
Type: article
Abstract: ABSTRACT Preserving the health of the mitochondrial network is critical to cell viability and longevity. To do so, mitochondria employ several membrane remodeling mechanisms, including the formation of mitochondrial-derived vesicles (MDVs) and compartments (MDCs) to selectively remove portions of the organelle. In contrast to well-characterized MDVs, the distinguishing features of MDC formation and composition remain unclear. Here we used electron tomography to observe that MDCs form as large, multilamellar domains that generate concentric spherical compartments emerging from mitochondrial tubules at ER-mitochondria contact sites. Time-lapse fluorescence microscopy of MDC biogenesis revealed that mitochondrial membrane extensions repeatedly elongate, coalesce, and invaginate to form these compartments that encase multiple layers of membrane. As such, MDCs strongly sequester portions of the outer mitochondrial membrane, securing membrane cargo into a protected domain, while also enclosing cytosolic material within the MDC lumen. Collectively, our results provide a model for MDC formation and describe key features that distinguish MDCs from other previously identified mitochondrial structures and cargo-sorting domains. SUMMARY Wilson and colleagues use electron tomography and time-lapse fluorescence microscopy to observe that mitochondrial-derived compartments (MDCs) are generated from outer mitochondrial membrane extensions that repeatedly elongate, coalesce, and invaginate to secure membrane cargo and cytosol within a distinct, protected domain.
Source: The Journal of Cell Biology
Institutions: University of Utah, University of Colorado Boulder
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1209485679, Manipulating Cell Metabolism via the Mitochondrial Derived Compartment Pathway, Training Program in Genetics, G6627015593, Endolysosomal Regulation of Amino Acid Homeostasis in Aging, Membrane trafficking to lysosomes, Investigating the Mitochondrial-Derived Compartment Pathway, Training in Cardiovascular Research
OpenAlex ID: W4401558348