Year: 2024
Type: article
Abstract: Vibrio species are significant pathogenic bacteria found in fish that induce gastroenteritis, a major public health issue.The present research investigated the occurrence, virulence gene detection, antibiotic resistance profile, and the antibacterial influence of lemon juice and pomegranate peel extract on V. cholerae and V. parahaemolyticus.Samples were 150 fish (30 of each Nile tilapia, Nile perch, sardine, Mugil cephalus, and Sea bass) from fish supermarkets.Sardine samples had the highest Vibrio count (4.77±1.22log 10 CFU/g), followed by and Sea bass (4.62±1.35log 10 CFU/g), Mugil cephalus (3.50±1.74log 10 CFU/g), Nile perch (3.24±1.05log 10 CFU/g), while Nile tilapia had the lowest count (2.90±0.78log 10 CFU/g), and 42% of all fish tested positive for Vibrio species.The most prevalent isolated Vibrio species were V. cholerae (38.2%),V. parahaemolyticus (31.7%),V. vulnificus (19%), and V. fluvialis (11.1%).According to a polymerase chain reaction analysis, most Vibrio isolates had one or more virulence genes.Antibiotic sensitivity tests showed that the isolates were highly resistant to many commonly used antimicrobial drugs in Egypt.These drugs included ampicillin, tetracycline, and sulfamethoxazole.The average MAR score was 0.530 indicating that the isolates have acquired a hereditary resistance that poses a public health hazard to customers.Moreover, the marinate with 5% lemon juice (LJ) and 3% pomegranate peel (PPE) extract had a positive impact on the diminution of V. cholerae and V. parahaemolyticus counts during the storage period by 48.47% and 37.3% for the LJ solution and by 41.51 and 40.13% for the PPE.It exhibited the most significant improvement in sensual characteristics.As a result, there is a need to authorize hygienic measures to regulate fish contamination in aquatic environments and markets.Additionally, marinating with natural components in food is a common approach to control Vibrio spp. with the primary objective of safe consumer health.
Authors: Sohaila Fathi El-Hawary, Nermeen M.L. Malak, Reda A. Gomaa, Hesham Z. Tawfeuk, Suzan Ismail, Nady Khairy Elbarbary
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
Funder: Aswan University
OpenAlex ID: W4401640253