Work

Comprehensive review of indicators and techniques for optical mapping of intracellular calcium ions

Year: 2024

Type: review

Abstract: Calcium ions (Ca2+) play crucial roles in almost every cellular process, making the detection of changes in intracellular Ca2+ essential to understanding cell function. The fluorescence indicator method has garnered widespread application due to its exceptional sensitivity, rapid analysis, cost-effectiveness, and user-friendly nature. It has successfully delineated the spatial and temporal dynamics of Ca2+ signaling across diverse cell types. However, it is vital to understand that different indicators have varying levels of accuracy, sensitivity, and stability, making choosing the right inspection method crucial. As optical detection technologies advance, they continually broaden the horizons of scientific inquiry. This primer offers a systematic synthesis of the current fluorescence indicators and optical imaging modalities utilized for the detection of intracellular Ca2+. It elucidates their practical applications and inherent limitations, serving as an essential reference for researchers seeking to identify the most suitable detection methodologies for their calcium-centric investigations.

Source: Cerebral Cortex

DOI: 10.1093/cercor/bhae346

Authors: Chu-Qiao Sheng, Shuang-Shuang Wu, Yong-Kang Cheng, Yao Wu, Yu-Mei Li

Institutions: Jilin University, First Hospital of Jilin University, State Key Laboratory of Supramolecular Structure and Materials

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Jilin University, Department of Science and Technology of Jilin Province

Award: G6301738293