Work

Irreversible Inhibition of Photophosphorylation by Low Temperature in Rhodospirillum Rubrum Chromatophores

Year: 1982

Type: article

Abstract: Photophosphorylation of Rhodospirillum rubrum chromatophores at low temperature produces an inhibition for this reaction of the order of 50% evidenced by the appearance of a breaking point in the time course curve. The presence of phenazine methosulfate (PMS) makes this inhibition irreversible. Dissipation of energy by the addition of an uncoupler (FCCP) or a lesser energy accumulation (lowering light intensity) produces a retard in the appearance of the breaking point without any change in the initial velocity. Mg-ATPase activity shows a slight or no change in specific activity, although a noticeable change in the susceptibility to FCCP is observed. These results are discussed connected to possible structural changes of the ATPase complex produced as a consequence of a diminished capacity of the ATP synthesizing system to dissipate energy as ATP.

Source: Photobiochemistry and photobiophysics.

DOI: 10.1016/s0165-8646(24)00070-9

Authors: Javierfernández Velasco, Oscar Romero, Normal Pucheu, Augustof Garcia

Institutions: Consejo Nacional de Investigaciones Científicas y Técnicas, Fundación Miguel Lillo

Language: English

Topic: Photosynthetic Processes and Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W4402016264

FWCI: 0.426

Cites: 21

Cited by: 3

Related to: 10