Protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior
Year: 2024
Type: article
Abstract: Fiber photometry technique allows investigation of in vivo neural activity during behavior allowing understanding of brain-behavior relationship. Here, we provide a protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior. We explain the detailed steps for stereotaxic virus injection, optic fiber cannula implantation, setup for synchronized fiber photometry and behavioral recording, and analysis of photometry data. These protocol steps can be adapted for various animal models, photometry, and behavioral recording systems. For complete details on the use and execution of this protocol, please refer to Tamboli et al. 1 and Amalyan et al. 2 • Step-by-step instructions for viral injections and optic fiber cannula implantation • Detailed setup and calibration guidelines for the wireless fiber photometry system • Configuration for synchronization of fiber photometry and behavioral video recordings • Comprehensive guidance on fiber photometry recordings, data extraction, and analysis Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Fiber photometry technique allows investigation of in vivo neural activity during behavior allowing understanding of brain-behavior relationship. Here, we provide a protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior. We explain the detailed steps for stereotaxic virus injection, optic fiber cannula implantation, setup for synchronized fiber photometry and behavioral recording, and analysis of photometry data. These protocol steps can be adapted for various animal models, photometry, and behavioral recording systems.
Source: STAR Protocols
Institutions: Centre hospitalier de l'Université Laval, Université Laval
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4403538575