Year: 2023
Type: preprint
Abstract: This protocol describes isolation and culture of basal cells from the olfactory epithelium of mice. The process involves serial enzymatic treatment of dissected olfactory epithelia and subsequent plating in commercial airway medium with dual SMAD inhibitors. The resulting cultures express the horizontal basal cell markers CK5 and CK14.
Authors: Alp Ozgun, Priya Suman, Josée Coulombe, Julianna Tomlinson, Earl G. Brown, John M. Woulfe, Michael G. Schlossmacher
Institution: Ottawa Hospital Research Institute
Language: English
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4403560730