Phytochemical Screening and Evaluation of Antioxidant and Cytotoxic Activities of Ganoderma lucidum
Year: 2024
Type: article
Abstract: This study aims to conduct a comprehensive investigation of the phytochemical composition of Ganoderma lucidum and evaluate its potential, antioxidant, and cytotoxic properties found in the higher altitude region of Nepal.Extraction was perform by maceration, Soxhlet extraction and maceration followed by Soxhlet using ethanol as solvent.The phytochemical screening was performed by standard method.Phytochemical screening of extract reveals that extract contains proteins, carbohydrates, terpenoids, saponins, glycosides, alkaloids.Antioxidant test was performed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxicity test was done by using Brine shrimp lethality assay.Among three methods of extraction, maceration process observed to have least extractive value and Soxhlet and Soxhlet followed by macerationseems increasing extractive value.Result shows that The IC50 of DPPH scavenging assay was determined as 53.88±0.62 and for cytotoxicity LD50 value was155.89±1.75.This research reveals that ganoderma show pronounced antioxidant and cytotoxic activity.The findings could explain why G. lucidum extracts are used in folk medicine.
Authors: Anil Bhusal, Santosh Dhungana, Sujan Sharma, Suraj Dahal, Krishna Neupane, Sajan Maharjan
Institutions: Pokhara University, CiST College
Language: English
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funder: Pokhara University
OpenAlex ID: W4403574673