Figure 4 from Targeting PRMT1 Reduces Cancer Persistence and Tumor Relapse in EGFR- and KRAS-Mutant Lung Cancer
Year: 2025
Type: other
Abstract: Chromosome 5q31.1 deletion is negatively associated with the effectiveness of the PRMT1-targeting strategy for reducing persistence. A and B, Chromosome 5q31.1 deletions in TCGA lung cancer cohorts. Proportions of LUAD and LUSC samples with (brown) or without (gray) deletion are shown in A. Proportions of samples with (brown) or without (gray) the deletion in LUAD harboring EGFR-activating mutations (EGFRmut) and KRASG12C mutations are shown in B. The numbers of samples with the deletion (brown) and the total cohort size (black) are labeled above the graphs. C, Box and whisker plots showing IRF1 protein levels in PERCs with or without the 5q31.1 deletion. Cells were treated with 50 ng/mL IFNγ for 2 hours. Protein levels were quantified from the Western blots in Supplementary Fig. S4C and normalized to the mean value across 17 PERCs. D, PRMT1 knockdown efficacy with PERCs. Cells were incubated with PRMT1-targeting siRNA (siPRMT1) for 2 days and then treated with 100 nmol/L osimertinib and 5 ng/mL IFNγ for 6 days. Persister fitness was calculated by normalizing cell viability to siNTC for each cell line. Data are shown as mean ± standard deviation (n = 3), ranked by the effect size of PRMT1 KD from large to small. Cell lines are color-labeled to indicate deleted (brown) or intact (gray) 5q31.1 region. E, Box and whisker plot showing PRMT1 knockdown efficacy between PERC groups with or without the 5q31.1 deletion. F, Distribution of t-statistics for PRMT1 knockdown efficacy between PERC groups with or without a copy number variation. Amplifications or deletions present in at least three PERC cell lines and no more than 14 PERC cell lines (i.e., absent in at least three PERC cell lines) were analyzed. Brown: the 5q31.1 deletion. Statistical comparison between groups was based on two-sided Student t tests assuming unequal variance. The significance of the results is indicated by symbols: *, P ≤ 0.05; **, P ≤ 0.01.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4406630786