Practical Use of the Chorioallantoic Membrane (cam) Model in the Creation of a Cryobank of Generative Cells of Female Gallus Gallus Domecticus
Year: 2025
Type: article
Abstract: One of the promising areas for preserving female reproductive cells of the Aves class is the use of embryonic tissues of reproductive organs and cells. Evaluation of the effectiveness of freezing methods for female reproductive organs of birds should be based on simplicity and availability. The chicken chorioallantoic membrane (CAM) model can be an accessible and effective approach to assessing the quality of frozen/ thawed avian ovarian tissues for subsequent orthotopic transplantation. The aim of the study was to determine the age of chicken embryos for obtaining sex-differentiated gonads and to evaluate the viability of transplanted embryonic female gonads on the chorioallantoic membrane by their neovascularization in the mesenchyme for further use of these screening methods in cryopreservation of embryonic female gametes of chickens. To obtain embryonic female gonads, fertilized eggs of Russian White chickens were used after incubation for 9 days - 10 pcs, and for 17 days - 10 pcs (in 3 replicates). The number of vascularized donor gonads after transplantation to the recipient's CAM from 9-day-old embryos was 75.0% of the total number of transplants; from 17-dayold embryos it was 63.6%. The presented data are intended for practical evaluation of the model of transplantation of donor embryonic ovarian tissue using the recipient's CAM for the purpose of its subsequent use in assessing the effectiveness of the vitrification protocol for embryonic gonadal tissues of chickens, as well as in the protocol for obtaining gonadal germ cells (GGCs) from embryonic ovarian tissues of chickens from 9-day-old embryos and transplantation of embryonic ovarian tissues from 17-day-old-embryos
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4406686232