Role of VDAC1 in hepatocyte apoptosis during acute liver injury in rats induced by obstructive jaundice.
Year: 2025
Type: article
Abstract: Objectives: Exploring the role of VDAC1 in hepatocyte apoptosis during acute liver injury induced by obstructive jaundice. Materials and Methods: Animal and cell models were established to investigate possible mechanisms during acute liver injury induced by OJ. Blood was collected for liver function assessment. H&E and TEM were employed to observe pathological changes in the liver tissues. Flow cytometry was used to measure the hepatocyte apoptosis. The mitochondrial MPTP assay was employed to assess the mitochondrial function of hepatocytes. IHC, western blot, and qRT-PCR were employed to determine the expression levels of VDAC1. Then, VDAC-siRNA was used to establish a knockdown model. Flow cytometry was used again to measure hepatocyte apoptosis following VDAC1 knockdown. Results: . Significant increases in the rates of hepatocyte apoptosis, particularly early apoptosis, were observed in the OJ groups. However, there was a reverse in the rates of hepatocyte apoptosis after knockdown regulation of VDAC1 only within the cells of the OJ group. Conclusion: The up-regulation of VDAC in liver injury caused by obstructive jaundice may lead to increased early apoptosis of hepatocytes.
Source: PubMed Central
Authors: Jinshan Liu, Jinlong Hu, Hongyu Xu, Liang Yan, Jiaming Yao, Baoqiang Cao
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4406964085