Year: 2025
Type: review
Abstract: R-loops are three-stranded non-canonical nucleic acid structures composed of nascent RNA hybridized with the template DNA strand, leaving the non-template DNA strand displaced. These structures play crucial roles in regulating gene expression, DNA replication, and transcription processes. However, R-loops have also been increasingly described as highly deleterious, causing genomic instability and DNA damage. To maintain R-loops at a relatively safe level, complex regulatory mechanisms exist to prevent their excessive formation. The growing understanding of R-loop functions has provided valuable insights into their structure and potential clinical applications. Emerging research indicates that R-loops contribute to the pathogenesis of various disorders, including neurodegenerative, immune-related, and neoplastic diseases. This review summarizes R-loop metabolism and its significance in the etiology of associated disorders. By elucidating the regulatory mechanisms governing R-loops, we aim to establish a theoretical foundation for understanding disease pathogenesis and exploring novel therapeutic strategies targeting these hybrid nucleic acid structures.
Authors: Junzhe Liu, Fengze Li, Yulong Cao, Yonghui Lv, Kunjian Lei, Zewei Tu, Chuandong Gong, Haiyan Wang, Feng Liu, Kai Huang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4407828657