Work

Protocol forIdentification of Genetic Variation in Genes Linked to Buparvaquone Resistance in Theileria spp. infecting dairy cattle in India v1

Year: 2025

Type: preprint

Abstract: Buparvaquone (BQO) is the primary drug used to treat bovine theileriosis, a tick-borne disease caused by T. annulata and T. orientalis. This protocol establishes a sensitive and rapid multiplex PCR-based method, combined with Oxford nanopore sequencing, for species detection and BQO resistance profiling of T. annulata and T. orientalis by targeting three key genes: cytochrome b (cytb), dihydroorotate dehydrogenase (dhodh), and peptidyl-prolyl isomerase (pin1), Monitoring and establishing the link between genetic variations in these genes and BQO resistance is crucial for understanding emerging resistance patterns and improving treatment strategies for bovine theileriosis.

DOI: 10.17504/protocols.io.4r3l29zk4v1y/v1

Authors: Pankaj Musale, Ajinkya Khilari, Bhagya Turakani, Dhanasekaran Shanmugam

Institutions: National Chemical Laboratory, Council of Scientific and Industrial Research, Academy of Scientific and Innovative Research, BAIF Development Research Foundation

Language: English

Topic: Vector-borne infectious diseases

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: BAIF Development Research Foundation

OpenAlex ID: W4409208752

Cites: 1

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Related to: 10