Synthesis, Characterization and Novel Antidiarrheal Investigations of Ipomoea Carnea Nanoparticles
Year: 2022
Type: article
Abstract: The research focused on authenticating and characterizing Ipomoea carnea leaves and bark, utilizing aqueous extracts for the green synthesis of phytonanoparticles.The macroscopic, organoleptic, and physicochemical analyses confirmed the plant's physical properties and material stability.The synthesis process was optimized, yielding well-dispersed nanoparticles, confirmed through TEM, SEM, and UV-VIS spectroscopy, exhibiting ideal particle size and stability.FTIR spectroscopy revealed key functional groups, such as hydroxyl, carboxyl, and phenolic groups, responsible for stabilizing the nanoparticles.Zeta potential analysis showed values ranging from -30 to -40 mV, indicating strong colloidal stability.Biological testing demonstrated the antimicrobial efficacy of IC-Ag-NP, particularly against E. coli and Salmonella species, with significant zones of inhibition and low minimum inhibitory concentrations.Cytotoxicity studies revealed that IC-Ag-NP was non-toxic to Caco-2 cells, maintaining viability rates above 90% at concentrations up to 100 µg/mL, confirming its biocompatibility.In vivo models, including castor oil-induced diarrhea, the charcoal meal test, and the enteropooling assay, demonstrated the antidiarrheal potential of IC-Ag-NP.The nanoparticles showed a dose-dependent reduction in diarrhea, intestinal motility, and fluid secretion, possibly due to anticholinergic or antihistaminic effects.This study provides a foundation for the development of IC-Ag-NP as a biocompatible, stable, and effective antibacterial and antidiarrheal agent for therapeutic use.
Source: European Chemical Bulletin
Author: Vaishali D. Naphade
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $107
OpenAlex ID: W4409405993