Investigation of antibacterial activity against some Vibrio strains of red Areca nut (Cyrtostachys renda) ethanol extract
Year: 2025
Type: article
Abstract: The Arecaceae family is known for its medicinal properties containing many natural substances with antibacterial activity. In this study, the seeds of red Areca (Cyrtostachys renda) belonging to the Arecaceae family were extracted from ethanol to preliminarily survey the chemical composition and to evaluate the antibacterial ability of the species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. All these species cause acute hepatopancreatic necrosis disease (AHPNS) and white feces disease (WFD) in shrimp. The research results showed that the red Areca nut extract contains active ingredients such as phenol, tannin and flavonoid. The minimum inhibitory concentration (MIC) of the red Areca nut extract against V. cholerae, V. parahaemolyticus and V. vulnificus was 12.50 mg/ml, 3.13 mg/ml and 6.25 mg/ml respectively and the minimum bactericidal concentration (MBC) was 200.00 mg/ml, 16.67 mg/ml and 10.42 mg/ml respectively. The results of the survey on the increase of V. cholerae, V. parahaemolyticus and V. vulnificus in TSA medium supplemented with red Areca nut ethanol extract over time (Time-kill curve assay) showed that red Areca nut extract had the best resistance to V. parahaemolyticus strain and completely killed V. parahaemolyticus at MIC concentration (3.13 mg/ml) after 2 hours of culture in living medium. Next, at 0.25MIC concentration (1.56 mg/ml), red Areca nut extract could completely kill V. vulnificus after 4 hours of culture in liquid medium. Finally, at MIC concentration (12.50 mg/ml), red Areca nut extract has the ability to completely destroy V.cholerae after 2 hours of culture in liquid medium.
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4409465111