Work

Single cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures

Year: 2025

Type: article

Abstract: Cellular heterogeneity is a common issue in differentiation protocols of oligodendrocytes (OLs) from human induced pluripotent stem cells. Our previous work described a novel method to generate OLs and highlighted the presence of glial progenitors. Here, we unravel the glial heterogeneity and characterize the response of isolated subpopulations to differentiation. This study provides a novel tool for studying the dynamics of glial development in vitro and on a transcriptomic level.

Source: npj Systems Biology and Applications

DOI: 10.1038/s41540-025-00515-z

Authors: Gabriela J. Blaszczyk, Valerio E. C. Piscopo, Taylor M. Goldsmith, Alexandra Chapleau, Julien Sirois, Geneviève Bernard, Jack P. Antel, Thomas M. Durcan

Institutions: Montreal Neurological Institute and Hospital, McGill University, McGill University Health Centre, Research Institute of the McGill University Health Centre

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,490

Funders: McGill University, ALS Society of Canada, Fondation Brain Canada, Canada First Research Excellence Fund, Canadian Institutes of Health Research, Fonds de Recherche du Québec - Santé

Awards: G5004838387, G659563360, G6910506812, G7176773705, G7642257574, G7863478369

OpenAlex ID: W4409470661

FWCI: 0.896

Cites: 20

Cited by: 3

Related to: 10