Transcriptomic analysis of mitohormesis associated with lifespan extension in Caenorhabditis elegans
Year: 2025
Type: preprint
Abstract: Non-lethal exposure to mitochondrial stress has been shown to have beneficial effects due to activation of signalling pathways, including the mitochondrial unfolded protein response (UPR mt ). Activation of UPR mt restores function of the mitochondria and improves general health and longevity in multiple model systems, termed mitohormesis. In C. elegans , mitohormesis can be accomplished by electron transport chain inhibition, decline in mitochondrial translation, decreased mitochondrial import, and numerous other methods that activate UPR mt . However, not all methods that activate UPR mt can promote longevity. These and other studies have started to question whether UPR MT is directly correlated with longevity. Here, we attempt to address this controversy by unravelling the complex molecular regulation of longevity of the nematode under different mitochondrial stressors that induce UPR mt by performing RNA-sequencing to profile transcriptome changes. Using this comprehensive and unbiased approach, we aim to determine whether specific transcriptomic changes can reveal a correlation between UPR mt and longevity. Altogether this study will provide mechanistic insights on mitohormesis and how it correlates with lifespan of C. elegans .
Authors: Juri Kim, Naibedya Dutta, Gilberto Garcia, Ryo Higuchi-Sanabria
Institution: University of Southern California
Language: English
Subfield: Aging
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4409727143