Work

Enterovirus A71 3C protease small scale expression and purification protocol v1

Year: 2024

Type: preprint

Abstract: This protocol details the expression and purification of enterovirus A71 3C protease construct bearing a C-terminal His-tag at small scale (<6L).

DOI: 10.17504/protocols.io.36wgqndzogk5/v1

Authors: Korvus Wang, Michael Fairhead, Eleanor Williams

Institution: University of Oxford

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Award: AI-driven Structure-enabled Antiviral Platform (ASAP)

OpenAlex ID: W4409727299

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