Year: 2025
Type: preprint
Abstract: Antibodies devoid of light chains are a promising class of biotherapeutics. Computational methods that address these molecules are crucially needed to accelerate the traditional, long and expensive experimental process of their discovery. Inverse folding, wherein one is tasked to predict a sequence given molecular coordinates, is an established method in scaffold-based protein design. Here we develop an inverse folding method specific to nanobodies. We demonstrate its application in nanobody-engineering scenarios of enriching binders from next-generation sequencing experiments and novel binder design.
Authors: Dawid Chomicz, Bartosz Janusz, Sonia Wrobel, Pawel Dudzic, Adithya Polasa, Kyle Martin, Steven Darnell, Stephen R. Comeau, Konrad Krawczyk
Institution: Boehringer Ingelheim (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4410074675