Cytotoxicity and Phytochemical Screening of Heliotropium indicum L. Extract from Malita, Davao Occidental Philippines
Year: 2025
Type: article
Abstract: The cytotoxicity properties of Heliotropium indicum L. were evaluated using the Brine Shrimp Lethality Assay (BSLA) meanwhile qualitative phytochemical screening was also tested on its leaf extracts. Cytotoxicity was evaluated in terms of Lethality concentration which measures the ability of the extract to kill 50% of test animals in a specific observation period. Brine shrimps were tested in three different extraction solvents with four different concentrations;their toxicity values were computed and described using Clarkson’s toxicity. Probit analysis and LC50 were employed to measure the degree of toxicity of the brine shrimps after 6 and 24 hours of treatment. Results of the cytotoxicity assay showed that the decoction and aqueous extracts have the highest toxicity values. Phytochemical screening was assayed using the protocol of Shaikh and Patil (2020). The qualitative phytochemical assessment of Heliotropium indicum L. leaf extracts showed the presence of alkaloids, flavonoids, and saponins from decoction and aqueous extract. It indicated that bioactive components are present in these plants that could account for their pharmacological effects. Thus, the results show tentative support for its pharmacological claims and its utilization as an alternative to traditional medicine.
Authors: Leonel Lumogdang, Pebegen Guia, Huey Baltazar
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4410095099