Bacillus halodurans RSCVS-PF21 an alkaline protease producing bacteria from poultry farm soil
Year: 2025
Type: article
Abstract: The aim of the study was to investigate potent alkaline protease producing indigenous bacterial strains from alkaline soils of Vindhya region of Madhya Pradesh, India. Accordingly, isolation and screening of alkaline protease producing alkaliphilic bacteria was performed from different habitats of Vindhya region of Madhya Pradesh, India. Selection and identification of promising alkaline protease producing isolates, enzyme activity assay and partial characterization of crude alkaline protease filtrate were done. Many alkaline proteases producing bacterial isolates were isolated from poultry farm soils of Vindhya region, Madhya Pradesh, India. Out of several proteolytic isolates, most potent new bacterial strain was identified as Bacillus halodurans RSCVS-PF21 (Genbank Accession No. MT279908) based on phenotypic, biochemical and molecular characterizations. Crude alkaline protease was stable and effective at high temperature. It was also highly stable in different organic solvents, so it can be used in many industrial processes.
Authors: Rakesh Singh Chauhan, Rahasya Mani Mishra
Institution: Awadhesh Pratap Singh University
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4410240960