Year: 2017
Type: article
Abstract: Dopamine-2 receptor (D2R) is shown to be important in motor and memory consolidation. Some neuropsychiatry disorders are said to arise as developmental problems, hence, age-dependent D2R was studied in mice. The study was designed to show if there is the presence of D2R in mice motor cortex and hippocampus and if it changes as the animals grow with age. Four adult male and female mice were used for breeding pups used for the experiments. The animals were allowed to mate freely. At birth (P0) three pups were sacrificed by decapitation, brain was excised rinsed in normal saline and transferred to specimen bottle containing formal saline solution. Three pups sacrificed by cervical dislocation at postnatal day 14, 28 and 42 respectively were fixed transcardially using formal saline. The brain was excised immersion-fixed in formal saline. The brains were processed immunohistochemically to identify D2R in the motor cortex and hippocampus. Number of D2R expressing cells were counted using ImageJ software, and data were represented on a line graph to show age-related changes using GraphPad software V.5.0. D2R was expressed in the motor cortex from birth, hippocampus from P14 till P42. Expression of D2R peaks at P28 in the motor cortex and hippocampus and decline at P42
Source: NIgerian Journal of Neuroscience
Institutions: Afe Babalola University, University of Ilorin
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4411001265