Vernonia ambigua Alters the Expressions of some Antioxidant Enzymes, Pro-Inflammatory Cytokines, and Acetylcholinesterase in the Cerebellar Cortex of Rats
Year: 2025
Type: article
Abstract: The therapeutic effects of medicinal plants are often attributed to their recognised and scientifically validated phytochemical constituents. However, literature is rare on the effects of Vernonia ambigua (VA), a documented medicinal plant, on the expression of antioxidant enzymes, cytokines, and neurotransmitters. This study aimed to examine the relative gene expression of cerebellar catalase (rCCAT), glutathione peroxidase-1 (rCGPx-1), tumour necrosis factor-alpha (rCTNF-α), interleukin-1 beta (rCIL-1β), and acetylcholinesterase (rCAChE) in male Wistar rats after the ingestion of an ethanol leaf extract of VA (ELEVA). The 200-220 g rats were divided into four groups, each containing five rats. The control group received feed and water, while test groups ELEVA (E)1, E2, and E3 received 100 mg/kg, 200 mg/kg, and 400 mg/kg of body weight of ELEVA, respectively, for 14 days. On the 15th day, portions of fresh cerebellar tissues were collected for total ribonucleic acid quantification and analysis using a spectrophotometer. Complementary deoxyribonucleic acid was produced using reverse transcriptase kits. rCTNF-α was significantly increased (p<0.05) in E1, E2, and E3, while rCGPx-1 and rCCAT were significantly increased and decreased, respectively, in E3. No significant difference (p>0.05) in rCIL-1β was observed in the test groups. The rCAChE in E1 and E2 were significantly reduced. ELEVA demonstrated concentration-dependent effects that reduced rCCAT and rCAChE but increased rCGPx-1 and rCTNF-α. Consequently, ELEVA may decrease AChE activity, offering potential therapeutic benefits while posing a risk of overstretched inflammatory responses. Additionally, the antioxidant effects of ELEVA may rely on elevated rCGPx-1, as it reduced rCCAT.
Source: NIgerian Journal of Neuroscience
Author: Emeka C. Igwe
Institution: Ebonyi State University
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4411234601