Development and interlaboratory validation of a cultivar-specific identification method for the table grape ‘Shine Muscat’ using loop-mediated isothermal amplification (LAMP)
Year: 2025
Type: article
Abstract: ‘Shine Muscat’ is an elite table grape cultivar developed by the National Agriculture and Food Research Organization in Japan. Recently, the infringement of breeders’ rights in various fruits has become a serious problem in Japan. In this study, a loop-mediated isothermal amplification (LAMP)-mediated cultivar identification method for ‘Shine Muscat’ was developed. We comprehensively analyzed retrotransposon insertion sites using 24 major grape cultivars and identified two insertions, VINE1-Cl160 and VINE1-Cl155, which are unique to ‘Shine Muscat’. LAMP primers targeting VINE1-Cl160 and VINE1-Cl155 were designed, and specific amplifications were confirmed. We also designed a primer set to detect the grape endogenous reference sequence, UDP-glucose:flavonoid 3-O-glucosyltransferase. To improve rapidness and cost-effectiveness, we applied single-stranded tag hybridization on a chromatography printed-array strip system, a lateral flow DNA chromatography technology. The developed method was validated with an interlaboratory study. This novel identification method would be particularly useful for border inspections.
Source: Ikushugaku zasshi
DOI: 10.1270/jsbbs.24074
Authors: Reona Takabatake, Yuki Monden, Akiko Shindo, Yasutaka Minegishi, Fumiya Taniguchi, Yu Hashimoto, Tomoyuki Takeuchi, Kazuto Takasaki, Sachiko Isobe
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4411477113