A novel “chassis” PSC line for precise safe harbor site integration via selection-aided RMCE
Year: 2025
Type: article
Abstract: We describe here a human pluripotent stem cell line (hPSC) designed to serve as an acceptor (a "chassis" cell line) for the recombination-mediated cassette exchange (RMCE)-mediated precise insertion of DNA fragments into a safe harbor locus. To enable the use of Flp recombinase for directional insertion, we targeted one of the AAVS1 safe harbor alleles with an excisable negative selection cassette flanked with FRT and FRT3 sites. The generated hPSC line displayed typical colony morphology, pluripotency signatures and normal karyotype. We expect it to provide a useful AAVS1 safe harbor "landing site" for FRT/FRT3-flanked transgenes.
Source: Stem Cell Research
Authors: Merve Ay, Desi Veleva, Mohammad M. Chowdhury, Andrew B.J. Prowse, Dmitry A. Ovchinnikov
Institutions: The University of Queensland, The University of Melbourne
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,360
OpenAlex ID: W4411582012