Work

Anti‐Inflammatory and Anti‐Hyperalgesic Effects of Ximenia americana Bark Extract in a Murine Model of Rheumatoid Arthritis

Year: 2025

Type: article

Abstract: The aim of this study was to evaluate the anti‐hyperalgesic and anti‐inflammatory profiles of the hydroethanolic extract of Ximenia americana bark (HEXA) in a murine model of rheumatoid arthritis (RA). The RA model was induced through intra‐articular injection of Complete Freund's Adjuvant (CFA). ELISA was used to analyze the production of inflammatory mediators, and nociception was assessed using the Von–Frey method. HEXA significantly reduced CFA‐induced mechanical hyperalgesia ( p < 0.01) and ipsilateral knee edema ( p < 0.001) These effects were associated with reduced levels of IL‐6 and TNF‐α ( p < 0.01) and increased IL‐10 concentrations ( p < 0.01) in the knee joint. HEXA also reduced total protein levels ( p < 0.0001) and myeloperoxidase (MPO) enzyme activity ( p < 0.0001) compared to untreated controls. Liver enzyme analysis revealed no signs of toxicity after chronic treatment. HEXA demonstrated significant anti‐hyperalgesic and anti‐inflammatory activity in a CFA‐induced arthritis model, supporting its potential as a candidate for further investigation in inflammatory joint diseases.

Source: ChemistrySelect

DOI: 10.1002/slct.202501146