Computational and In Vitro Exploration of Antioxidant and Anti-inflammatory Potential of Clitoria ternatea White-Flower Leaf Extract
Year: 2025
Type: article
Abstract: Aim: This study evaluates the therapeutic potential of Clitoria ternatea (White flower plant) ethanolic leaves extract using an in-silico approach, focusing on its antioxidant and Anti-Inflammatory Properties.Materials and Methods: Ethanolic extraction was performed using the Soxhlet method, followed by qualitative analysis, including phytochemical, FT-IR, and GC-MS analyses, as well as total flavonoid, total phenolic, and terpenoid content analyses.Predict the therapeutic potentials of the extract with PDB ID: 3VLN (Antioxidant), PDB ID: 2AZ5 (Anti-inflammatory), and validate the in-silico study by performing the respective in vitro study.Results: Flavonoids, anthocyanins, saponins, phenolic compounds, and terpenoids are bioactive compounds in the extract, and their functional groups are reported by phytochemical tests and FTIR, respectively.GC-MS identifies 43 different molecules.Total flavonoid content is 0.0153 mg of Quercetin QUE /gm extract, total phenolic 0.0182 of GAE/gm extract, and terpenoid content is 96.5%.Molecular docking study predicted 14 molecules for antioxidant activity, 14 molecules for anti-inflammatory activity, and 21 molecules for antimicrobial activity.Extract and ascorbic acid % Radical Scavenging Assay at 300 µg/mL are 57.16±0.48 and 39.15±0.62%,respectively.The IC₅₀ values for the extract and ascorbic acid are 171.35µg/mL and 120.81 µg/mL, respectively.The HRBC membrane stabilization assay showed % Protection at concentrations of 1000 μg/ml of extract (53.12±0.82%)and Aspirin (85.13±0.87%). Conclusion:The present research focuses on the therapeutic properties of Clitoria ternatea (white flower) ethanolic leaf extract, which is abundant in flavonoids, phenolics, and terpenoids.GC-MS, molecular docking, ADMET, and in vitro studies indicate its antioxidant and anti-inflammatory properties, indicating potential use in phytopharmaceuticals and functional food development.
Institution: Maulana Azad University Jodhpur
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4414768017