Neuroprotective Effect of Orostachys Spinosa (l.) Sweet Dry Extract in Cerebral Ischemia in Wistar Rats
Year: 2025
Type: article
Abstract: Intriduction. The neurodegenerative and cerebrovascular diseases are among the leading causes of disability all over the world and the second-largest risk factor for premature mortality. A perennial plant of the Crassulaceae family, Orostachys spinosa (L.) Sweet, deserves special attention in the prevention and comprehensive treatment of these diseases. Its aboveground parts are characterized by a metabolites diversity. The aim of the study – to evaluate the neuroprotective effect of Orostachys spinosa dry extract in cerebral ischemia/reperfusion in Wistar rats. Material and methods. Сerebral ischemia was modeled by a five-minute occlusion of the common carotid arteries on Wistar rats. O. spinosa extract at a dose of 100 mg/kg was administered for 14 days before modeling ischemia. After 48 hours the animals were tested in the "open field" and a conditioned passive avoidance reaction (CPAR) was developed. After 24 and 72 hours the neuron-specific enolase (NSE) level were determined in the blood serum and the growth factors (BDNF, GDNF and VEGFa) content in the brain cytolysate. Also histological studies of the brain were performed. Research results. It has been established that O. spinosa extract increases the rearings and the animal movements number in the "open field", and also improves the development and maintenance of the CPAR. O. spinosa extract decreases the content of NSE and increases the concentration of growth factors in the brain cytolysate. O. spinosa extract decreases the number of pyknotic neurons by 24 and 30% in the cerebral cortex and hippocampus, reduces the number of "shadow cells" by 20% (p < 0.05). Conclusion. O. spinosa dry extract has a neuroprotective effect during cerebral ischemia/reperfusion
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4415508286