Simultaneous quantification of quercetin and rifampicin in bronchoalveolar lavage fluid by HPLC-UV technique and its application to a pharmacokinetic study of investigational dry powder formulation
Year: 2025
Type: article
Abstract: A rapid and precise high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed for the quantitative determination of Rifampicin and Quercetin, in bronchoalveolar lavage fluid. The validation process was conducted in compliance with the USFDA guidelines. Separation was achieved using a C18-Luna Phenomenex column (5 μm, 4.60 × 150 mm) with a mobile phase comprising 20 mM disodium hydrogen orthophosphate (pH 4.5) and methanol in a 35:65 %v/v ratio. The retention times for Quercetin and Rifampicin were 5.42 and 8.60 minutes, respectively. Optimal protein precipitation was achieved using 500 μl of acetonitrile and 500 μl of methanol, yielding an extraction recovery exceeding 80 % for both drugs from bronchoalveolar lavage fluid. The extraction method yielded clean samples, rendering the process both straightforward and cost-effective. The method exhibited a strong linear correlation (r > 0.9995) between the peak area ratio and the concentrations for Rifampicin (50– 5000 ng/ml) and Quercetin (40–4000 ng/ml). Pharmacokinetic analysis revealed that poly(lactic-co-glycolic acid) nanoparticles sustained higher drug concentrations in the lung tissue for an extended period of up to 24 hours. In conclusion, the developed HPLC method, utilizing simple UV detection, offers several advantages, including costeffectiveness, high recovery rates, a broad linear range, robust quantification capability, and short analysis times.
Source: Journal of Research in Pharmacy
Institution: The Charutar Vidya Mandal (CVM) University
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4415549871