Work

Cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC fruit on B16F10 melanoma cell culture

Year: 2025

Type: article

Abstract: Introduction: This study aimed to investigate the cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC (ZA) fruit on B16F10 melanoma cell cultures.Material and methods: The ethyl acetate fraction was obtained through maceration followed by fractionation with n-hexane and ethyl acetate.Cytotoxicity was assessed using the MTT assay to determine the IC 50 value.B16F10 melanoma cells were then grouped into control, IC 50 , ¾ IC 50 , ½ IC 50 , and doxorubicin groups.Apoptosis induction was analysed using Annexin V and caspase-3 expression assays.Results: The ethyl acetate fraction of ZA fruit exhibited cytotoxic activity on B16F10 melanoma cells, with an IC 50 value of 249.3 µg/ml.Apoptosis induction was confirmed by a significant increase in caspase-3 expression compared to the control group (p < 0.05).Conclusions: The ethyl acetate fraction of ZA fruit demonstrated cytotoxic effects and induced apoptosis in B16F10 melanoma cells, potentially through caspase-3 activation.

Source: Współczesna Onkologia

DOI: 10.5114/wo.2025.155773

Authors: Eka Megawati, Hakim Bangun, Imam Putra, Muhammad Rusda

Institution: Universitas Sumatera Utara

Language: English

Topic: Phytochemical Studies and Bioactivities

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $215