Figure 3 from Endo180 (MRC2) Antibody–Drug Conjugate for the Treatment of Sarcoma
Year: 2025
Type: other
Abstract: A5/158 is suitable for intracellular drug delivery. A, Confocal images of sarcoma and epithelial cells cultured with A5/158-488 (green) for 1 hour at 4°C followed by incubation with LysoTracker Red (red) and Hoechst 33342 (blue) for 30 minutes at 37°C. White arrowheads indicate areas of green–red colocalization. Representative images out of two fields of view, from experiments repeated on at least 3 occasions with equivalent findings. Scale bar, 50 μm. B, Representative graphs of MG-63, HT-1080, and HT-29 cell growth (independent experiments; MG-63, n = 3; HT-1080, n = 2; HT-29, n = 1) treated with A5/158 alone, A5/158-sb-saporin, Isotype-sb-saporin, or saporin alone. MG-63 and HT-29 were cultured with 0.5 μg/mL of each treatment or molar equivalent of saporin alone for 144 hours. HT-1080 was cultured with 1.5 μg/mL of each treatment or molar equivalent of saporin alone for 72 hours (n = 3 wells per condition; mean values ±SEM). Cell growth measured by confluency on the IncuCyte. C, At endpoint, cell viability readouts were determined by CellTiter-Glo. Data are normalized to untreated cells (n = 3 wells per condition, mean values ± SEM).
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4416764643