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Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch‐down overlapping PCR coupled with HiFi long‐read sequencing

Year: 2025

Type: article

Abstract: Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.

Source: Journal of Integrative Plant Biology

DOI: 10.1111/jipb.70107

Authors: Jie Huang, Yu Cheng, Jing Ruan, Xixi Liu, Dandan Xia, Yiting Chen, Delong Fan, Jiezheng Ying, Yifeng Wang, Xiaohong Tong, Zhiyong Li, Dawei Xue, Jianwei Zhang, Jian Zhang, Yuxuan Hou

Institutions: China National Rice Research Institute, State Key Laboratory of Rice Biology, Hangzhou Normal University, Huazhong Agricultural University

Language: English

Topic: Bioinformatics and Genomic Networks

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,000

Funders: National Natural Science Foundation of China, Chinese Academy of Agricultural Sciences, Huazhong Agricultural University, National Science and Technology Major Project, Agricultural Science and Technology Innovation Program, Natural Science Foundation of Zhejiang Province

Awards: G2638564968, 水稻多性状协同调控耦合效应及育种利用评价, G4825268258, G5191408700, G5230090515, 水稻抗穗发芽基因OsPHSZ的分子机制研究

OpenAlex ID: W4417020163

FWCI: 0.221

Cites: 10

Cited by: 1

Related to: 10