Paraoxonase Activity and Gene Polymorphism in Rheumatoid Arthritis among Egyptians
Year: 2014
Type: article
Study design: Observational Study
Abstract: Paraoxonase1 (PON1) is an esterase enzyme that has antioxidant activity and is tightly associated with high density lipoprotein (HDL) in blood. Decreased activity of PON1 has been reported in many diseases and could be partially attributed to PON1 glutamine 192 arginine polymorphism. The aim of this study was to determine the paraoxonase (PON1) status, i.e.PON1 activities and phenotypes (QQ, QR and RR), and its relationship with lipid status and lipid peroxidation among Egyptians with rheumatoid arthritis (RA). The study included 40 RA patients and 40 healthy volunteers. The level of malondialdehyde (MDA), as a marker for oxidative stress, plasma paraoxonase 1 (PON1) and arylesterase (ARE) activities were determined using an enzymatic spectrophotometric method. PON1 192 gene polymorphism was determined using polymerase chain reaction based restriction fragment analysis. The results of the present work showed that PON1 and ARE activities were statistically lower (P <0.001) while the MDA level was found to be statistically higher (P<0.001) in the RA group compared with the control group. There was no significant difference in the genotype distribution between RA group and control group. No significant difference was observed between the levels of total triglycerides, total cholesterol, HDL-C and LDL-C in both groups. We concluded that PON1 and ARE activities, which have antiatherogenic capability, are decreased in patients with RA. PON1 and ARE activities may be affected by oxidative stress demonstrated by high MDA level which contribute to the pathogenesis of RA. PON1 192 Q/R polymorphism may not be associated with RA incidence in Egyptians.
Language: English
Subfield: Clinical Biochemistry
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W50031349