Year: 2010
Type: conference-paper
Abstract: Integrated dark and photo (two-stage) fermentation was employed to enhance the performance of H 2 production.First, the continuous dark fermentation using indigenous Clostridium butyricum CGS5 was carried out at 12 h HRT and fed with sucrose at a concentration of 18750 mg/l.The overall H 2 production rate and H 2 yield were fairly stable with a mean value of 87.5 ml/l/h and 1.015 mol H 2 /mol sucrose, respectively.In addition, a relatively high ammonia nitrogen content (574 mg/l) in the dark fermentation effluent was observed.The soluble metabolites from dark fermentation, consisting mainly of butyric, lactic and acetic acids, were directly used as the influent of continuous photo-H 2 production process inoculated with Rhodopseudomonas palutris WP 3-5 under the condition of 35 o C, 10000 lux irradiation, pH 7.0 and 48 h HRT.The maximum overall hydrogen production rate from photo fermentation was 16.4 ml H 2 /l/h, and the utilization of the soluble metabolites could reach 90%.The maximum H 2 yield dramatically increased from 1.015 mol H 2 /mol sucrose (in dark fermentation only) to 6.04 mol H 2 /mol sucrose in the combined dark and photo fermentation.Surprisingly, the operation strategy applied in this work was able to attain an average NH 3 -N removal efficiency of 92%, implying that our photo-H 2 production system has a higher NH 3 -N tolerance, demonstrating its high applicability in an integrated dark-photo fermentation system.
Source: JuSER (Forschungszentrum Jülich)
Authors: S. S. Cheng, Detlef Stolten, Thomas Grube, Yang Chao, Ming Bai, Yen‐Hui Lin
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W589404131