Single-Molecule Imaging Reveals Dynamics of CREB Transcription Factor Bound to Its Target Sequence
Year: 2015
Type: article
Abstract: Proper spatiotemporal gene expression is achieved by selective DNA binding of transcription factors in the genome. The most intriguing question is how dynamic interactions between transcription factors and their target sites contribute to gene regulation by recruiting the basal transcriptional machinery. Here we demonstrate individual binding and dissociation events of the transcription factor cAMP response element-binding protein (CREB), both in vitro and in living cells, using single-molecule imaging. Fluorescent-tagged CREB bound to its target sequence cAMP-response element (CRE) for a remarkably longer period (dissociation rate constant: 0.21 s(-1)) than to an unrelated sequence (2.74 s(-1)). Moreover, CREB resided at restricted positions in the living cell nucleus for a comparable period. These results suggest that CREB stimulates transcription by binding transiently to CRE in the time range of several seconds.
Source: Scientific Reports
DOI: 10.1038/srep10662
Institutions: The University of Osaka, RIKEN
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: The Mechanism Underlying the Formation of Activity-Dependent Thalamocortical Projection, Cytoskeleton mechanisms of activity-dependent axon branching, Dynamics of Nuclear Architecture and Genome Organization during Neuronal Differentiation, A study of human neuronal circuit formation using iPS cells, A study of DNA repair dynamics during neuronal differentiation using single-molecule imaging, A study of neuronal activity-dependent transcription factor dynamics using single-molecule imaging, A study of the molecular mechanism underlying activity-dependent thalamocortical axon branching, G6104886164
OpenAlex ID: W595677928