Амперометрический биосенсор, модифицированный многостеночными углеродными нанотрубками, для определения глюкозы
Year: 2012
Type: article
Abstract: To improve analytical characteristics of amperometric biosensors for glucose determination, aminated and carboxylated multiwalled carbon nanotubes were applied. Attested samples of nanotubes were suspended and used for modification of amperometric transducers С220АТ of DropSens production and bioselective membranes. The glucose oxidase membranes were formed on the surface of working electrode by several methods: covalent crosslinking and coimmobilization with horseradish peroxidase in bovine serum albumin gel in glutaraldehyde vapors; electropolymerization of poly(3,4ethylenedioxythiophene) in the conductive polymer film. Electrochemical measurements were performed using the device μStat 200 of DropSens production. The developed amperometric biosensors with a membrane with carbon nanotubes have been shown to be advantageous as compared to those without carbon nanotubes in terms of higher sensitivity, lower working potential, and wider dynamic range of the target analyte detection. The optimized biosensor was used for detection of glucose in real wine samples. A reliable correlation was observed between the results of biosensor method proposed and those obtained by high performance liquid chromatography.
Source: Biotechnologia Acta
Authors: Н. С. Рогалева, Л. В. Шкотова, О. В. Львова, В. В. Гарбуз, В. Б. Муратов, Т. И. Дуда, Aleksey Vasilev, Я. И. Корпан, О. А. Белоиван
Language: Russian
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W6861398