Seed Viability of Commiphora leptophloeos (Mart.) J.B. Gillett (Burseraceae) Assessed by the Tetrazolium Test
Year: 2025
Type: article
Abstract: Commiphora leptophloeos, a native Caatinga species with economic and medicinal potential, faces propagation challenges due to seed dormancy and extractive use. The germination test, the official method for seed quality assessment, is time-consuming, whereas the tetrazolium test (TZT) offers a rapid alternative for determining seed viability. This study aimed to establish and validate a TZT protocol for C. leptophloeos seeds. Seeds collected in 2025 were extracted after natural fruit drying and then stored in a cold chamber. The germination test was conducted with seeds without pyrenes at 30 °C. For the TZT, a completely randomized design was used in a 6 × 4 factorial scheme (six TZT concentrations × four immersion times), with adjustments in seed preparation and staining procedures. Higher concentrations (0.5% and 0.75%) combined with shorter immersion periods (2 h) provided the best results, especially 0.75% for 2 h, which yielded 89% viability. Very low concentrations combined with short periods resulted in little or no staining. Compared with the germination test (35%), the TZT showed greater sensitivity in detecting viable seeds. We conclude that the TZT is highly efficient for assessing the viability of C. leptophloeos seeds, with optimal responses at 0.5–0.75% TTC and 2–4 h immersion periods, and represents a strategic tool to support the conservation and sustainable use of this species.
DOI: 10.3390/ijpb16040139
Authors: Jamille Cardeal da Silva, Jailton de Jesus Silva, Raquel Araujo Gomes, Claudinéia Regina Pelancani Cruz, Barbara França Dantas
Institutions: Universidade Estadual de Feira de Santana, Embrapa Semiárido
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $1,448
Awards: G29316538, G7186631681
OpenAlex ID: W7109942579