Comparative effects of ciprofloxacin and prednisolone on renal function markers in a TNBS-induced Crohn's disease rat model
Year: 2025
Type: article
Abstract: Crohn's disease (CD) is a chronic inflammatory bowel disorder characterized by mucosal inflammation and systemic complications, including renal impairment. This study investigated the comparative effects of ciprofloxacin and prednisolone on renal function markers, electrolyte balance, oxidative stress, and renal histoarchitecture in a trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease rat model. Forty male rats were randomly assigned into four groups (n = 10 per group): Group A (normal control), Group B (TNBS-induced CD), Group C (CD + ciprofloxacin), Group D (CD + prednisolone). Serum creatinine, urea, and electrolyte levels (Na⁺, K⁺, Cl⁻) were measured, alongside kidney malondialdehyde (MDA) levels as a marker of oxidative stress. Histopathological changes were assessed via hematoxylin and eosin staining. TNBS induction showed significant increases in serum creatinine (0.40±0.01 to 6.27±0.66 mg/dL), urea (16.67±0.88 to 45.33±1.55 mg/dL), kidney malondialdehyde (MDA) levels (0.03±0.014 to 1.28±0.04 µmol/mL), and significant decrease in electrolytes; sodium (139.67±2.33 to 84.34±1.49 mmol/L) and chloride (100.33±2.33 to 54.66±1.70 mmol/L) when compared to the control (p < 0.05). Treatment with ciprofloxacin and prednisolone significantly improved these parameters, although prednisolone produced greater reductions in creatinine (1.30±0.02 vs 5.90±0.03 mg/dL), urea (23.33±0.97 vs 37.53±1.18 mg/dL), and MDA (0.17±0.03 vs 0.51±0.03 µmol/mL), alongside more substantial restoration of sodium and chloride levels when compared with ciprofloxacin (p < 0.05). Histological analysis showed partial preservation of glomerular and tubular structures with ciprofloxacin and more pronounced architectural protection with prednisolone. Both agents attenuated TNBS-induced renal injury, but prednisolone demonstrated superior renoprotective effects, likely due to stronger suppression of inflammation and oxidative stress.
Source: Kragujevac Journal of Science
Institution: Olabisi Onabanjo University
Language: English
Topic: Inflammatory Bowel Disease
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7116843475