Work

Influence of estrous cycle in chromatin configuration in germinal vesicle (GV) of canine oocytes

Year: 2013

Type: article

Abstract: In bitches, oocyte maturation rates are low and the percentage of oocytes that remain in germinal vesicle (GV) independent of culture conditions is high. The objective of this study is to evaluate the configuration and positioning of chromatin in GV oocytes from bitches in anestrus and diestrus phases. The ovaries were sliced and selected only the cumulus-oocyte complex degree (COCs) I. These were subjected to hyaluronidase solution of 2% and stained with Hoechst. Were identified four stages of chromatin configuration classified as GV-1, GV-2 and GV-3 and GV-4. The analyzed data showed a significant difference (p≤0,05) between the phases demonstrating greater proportion of GV-1 and GV-2 in anoestrus and GV-3 and GV-4 in the diestrus phase.

Source: LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)

Authors: Pereira, L. M.C. [UNESP], Bersano, P. R.O. [UNESP], Lopes, M. D. [UNESP]

Language: Portuguese

Topic: Reproductive Biology and Fertility

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W7120660552

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