Genetic basis and phenotypical manifestations of wound pathogens of the russian-ukrainian war
Year: 2025
Type: article
Study design: Observational Study
Abstract: Annotation. An important characteristic of gunshot wounds is the presence of microbial contamination, which always leads to the development of an infectious process. The full-scale aggression of the russian federation against Ukraine is marked by the use of modern weapons, the use of which leads to an increase in cases of soft tissue damage, a high degree of their contamination with the subsequent occurrence of purulent-inflammatory complications. Of particular concern is the emergence of a huge reservoir of multiresistant strains of microorganisms with a high potential for global spread. The purpose of our study is to summarize the results of our own observations on the species composition, phenotypic manifestations and genetic determinants of antibiotic resistance of microflora, which causes purulent-inflammatory complications of gunshot and mine-explosive wounds in victims of the hostilities of the Russian-Ukrainian war. We conducted a bacteriological study of the contents of combat wounds of 171 victims. Isolation of pure cultures of non-fermenting bacteria was performed by the plate method. Pure bacterial cultures were identified by morphological, cultural and biochemical characteristics. Determination of antibiotic susceptibility was investigated by the Kirby-Bauer test in accordance with the recommendations of EUCAST (European Committee on Antimicrobial Susceptibility Testing). To determine the determinants of antibiotic resistance and phylogenetic interstrain relationships, the full genome of the isolated cultures was sequenced by the next-generation sequencing method on the Applied biosystems/life technologies (SOLiD system) platform with subsequent multilocus typing of genome sequences. The results of the conducted studies established the appearance in the spectrum of wound pathogens of gram-negative bacteria A. baumannii, P. aeruginosa and K. pneumoniae, which belong to epidemic international clones of high risk (ICs) and are characterized by resistance to most modern clinically significant antibiotics: protected aminopenicillins, cephalosporins, fluoroquinolones, nitrofurans, carbapenems.
Language: English
Subfield: Microbiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7121108970