A Simple, Rapid, and Highly Sensitive Magnetic Beads ELISA for Detection of SARS CoV-2 antibodies (IgG) in Plasma Samples as a Point of Care Assay v1
Year: 2025
Type: preprint
Abstract: This work deals with the development of an ELISA using magnetic beads as a point of the care assay that is reproducible, highly sensitive, robust, and easy to perform. It was successfully used for the detection of NP-specific IgG antibodies in plasma samples. This assay can be used as a professional assay, where the results can be verified with an ELISA reader. This assay may also be suitable for small clinics under field conditions. It can be used to detect a SARS CoV-2 infection in vaccinated persons (S protein-based vaccines) as well as in the non-vaccinated population both in the latent and active phases. This ELISA will be completed within 20 minutes and is more than 100 times more sensitive than conventional ELISA. In addition, this can be used as a rapid test at the point of the care.
Authors: Sudhir Bhatia, Gudrun Baersch
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7123349598