Work

Improved Open Modification Searching via Unified Spectral Search with Predicted Libraries and Enhanced Vector Representations in ANN-SoLo

Year: 2026

Type: article

Abstract: The primary computational challenge in mass spectrometry-based proteomics is determining the peptide sequence responsible for generating each measured tandem mass spectrum. This task is traditionally addressed through sequence database searching as well as alternative approaches such as spectral library searching. ANN-SoLo is a powerful spectral library search engine optimized for open modification searching, enabling the detection of peptides carrying any post-translational modification. Here, we present an enhanced version of ANN-SoLo that combines the strengths of both spectral library searching and sequence database searching by integrating with Prosit to generate predicted spectral libraries from protein sequence databases. Additionally, it provides functionality to generate decoys at both the spectrum and the peptide levels, introduces an optimized internal file structure for large-scale analytics, and improves search accuracy by incorporating complementary ion information into spectrum vector representations. These advancements collectively address challenges associated with missing spectral libraries and enhance peptide identification in large-scale and complex proteomics workflows.

Source: Journal of Proteome Research

DOI: 10.1021/acs.jproteome.5c00797

Authors: Issar Arab, Kris Laukens, Wout Bittremieux

Institutions: University of Antwerp, Biomedical Informatics Research Center Antwerp

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: Fonds Wetenschappelijk Onderzoek

Award: Bioinformatics Solutions For the Comprehensive Study of the Human Immunopeptidome